A nurse quarantined in New Jersey after treating Ebola patients in Sierra Leone will be discharged after threatening legal action over her confinement.
Kaci Hickox said she was made to feel like a criminal after returning to the US last Friday.
She is free of symptoms and will be flown privately to her home state of Maine, New Jersey officials said.
The White House and mayor of New York have expressed concerns over new strict quarantine orders in several US states.
The new rules in New York, New Jersey and Illinois require a mandatory 21-day quarantine for all health workers who have had contact with Ebola patients in West Africa upon their return to the US.
The measures were announced after a New York doctor who had treated patients in Guinea fell ill with Ebola last week.
Liberia remains the worst affected country, with 4,665 cases Amid criticism the quarantine rules were overly strict, New York Governor Andrew Cuomo eased the state's restrictions on Sunday.
Now, returning health workers who have displayed no symptoms will be allowed to pass the quarantine period in their homes, will be allowed contact with their families and friends, and will be monitored twice daily. Compensation will be offered for lost earnings.
More than 10,000 people have contracted the Ebola virus, with 4,922 deaths, according to the World Health Organization's latest figures.
All but 27 of the cases have occurred inside Sierra Leone, Liberia and Guinea.
Separately on Monday, a five-year-old boy was being tested for Ebola at Bellevue Hospital in New York City after visiting an Ebola-stricken country and developing a fever, hospital officials said. Test results were expected later in the day.
In a statement on Monday, the New Jersey health department said Ms Hickox had tested negative for Ebola on Saturday and had been free of symptoms for 24 hours.
BBC's Anne Soy: "The world is dealing with two epidemics - the bigger one is that of fear"
Ms Hickox arrived at Newark Airport on Friday and was placed in isolation after developing a fever, the health department said.
"She was cared for in a monitored area of the hospital with an advanced tenting system that was recently toured and evaluated by the CDC.
"While in isolation, every effort was made to insure that she remained comfortable with access to a computer, cell phone, reading material and nourishment of choice."
Over the weekend, Ms Hickox, of medical charity Medecins Sans Frontieres (MSF), said she underwent hours of questioning at the airport before being transferred to a hospital isolation tent outside University Hospital in Newark.
She described the experience as "frightening" and a "frenzy of disorganisation".
New York Mayor Bill de Blasio said the treatment of Ms Hickox was inappropriate New Jersey Governor Chris Christie had defended the state's quarantine requirements.
Ms Wilcox's lawyer Norman Siegel said her isolation raised civil liberty issues given that she had displayed no Ebola symptoms and did not test positive for the virus.
"We're not going to dispute that the government has, under certain circumstances, the right to issue a quarantine," he said, adding that "the policy is overly broad when applied to her".
New York Mayor Bill de Blasio has described Ms Hickox as a "returning hero", but said that she had been "treated with disrespect" when put into quarantine.
"We owe her better than that and all the people better than that," he told reporters outside New York's Bellevue Hospital, where an American doctor, Craig Spencer, is being treated for the virus.
The stricter measures were put in place after Dr Spencer tested positive for Ebola on his return from Guinea last week and unwittingly moved around the city before the symptoms developed.
Workers clean the apartment of Dr Craig Spencer, currently being treated for Ebola in New York City The White House has warned that such restrictions could put off aid workers and others travelling to West Africa to help mitigate the crisis at its source.
Meanwhile, the US ambassador to the UN criticised the level of international support for nations hit by Ebola.
Some nations who had offered backing "haven't taken responsibility yet" in supplying aid and doctors, Samantha Power said shortly before arriving in Guinea.
Ms Power last week said the international community "isn't just losing the race to Ebola. We are getting lapped", and even praised Cuba - under a US embargo for decades - for its supply of doctors to Sierra Leone.
The US has pledged 4,000 troops to build hospitals and to train health workers in West Africa, some 600 of whom have already arrived.
How Ebola survivors' blood is saving lives
- Symptoms include high fever, bleeding and central nervous system damage
- Spread by body fluids, such as blood and saliva
- Fatality rate can reach 90% - but current outbreak has mortality rate of about 70%
- No proven vaccine or cure
- Fruit bats, a delicacy for some West Africans, are considered to be virus's natural host
Discharge for quarantined US nurse
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